The development of melanoblasts from leg bud mesenchyme grown in the celom of chick embryos

Presence of extra-epidermal melanoblasts in the messenchyme of leg bud have been acertained. Small pieces of 0.2 mm3 of isolated mesoderm of outer and inner core, from leg bud of 5 day Silver Campine, grafted into celom of 3 day White Leghorn gave positive results. The viability and migratory capacity of these cells have been practically examined. The migration of pigment cells into the hind limb musculature; differentiation of melanoblasts in different tissue environments and the behaviour of grafted melanoblasts in the host body is discussed.