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Identification of the corticosteroid-sensitive cells in antibody responses in mice
To elucidate the cellular basis of corticosteroid-induced suppression of antibody response, the immune responses of hydrocortisone-pretreated mice against various antigens, both thymus-dependent and thymus-independent antigens, were compared with those of normal mice. The in vitro antibody responses to some antigens were also studied by a combination of lymphocyte subpopulations and macrophages from normal and hydrocortisone-treated mouse spleen. It was shown that the antibody responses to all antigens tested were significantly reduced by hydrocortisone. Experimental results in vitro also suggested that the susceptibility of splenic B cells to hydrocortisone treatment was higher than that of T cells, although the helper T cell function was significantly suppressed by hydrocortisone. No impairment of macrophage functions by hydrocortisone was observed in our experiments.
To elucidate the cellular basis of corticosteroid-induced suppression of antibody response, the immune responses of hydrocortisone-pretreated mice against various antigens, both thymus-dependent and thymus-independent antigens, were compared with those of normal mice. The in vitro antibody responses to some antigens were also studied by a combination of lymphocyte subpopulations and macrophages from normal and hydrocortisone-treated mouse spleen. It was shown that the antibody responses to all antigens tested were significantly reduced by hydrocortisone. Experimental results in vitro also suggested that the susceptibility of splenic B cells to hydrocortisone treatment was higher than that of T cells, although the helper T cell function was significantly suppressed by hydrocortisone. No impairment of macrophage functions by hydrocortisone was observed in our experiments.