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Immunoglobulin class commitment exhibited by B lymphocytes separated according to surface isotype

J M Teale, D Lafrenz, N R Klinman

    Journal of Immunology (Baltimore, Md. : 1950)
    |May 1, 1981
    PubMed
    Summary
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    The regulation of antibody class expression.

    Immunology today·2014

    This study reveals that most primary B cells can produce multiple antibody classes, while memory B cells show varied class commitment. Surface immunoglobulin mu (IgM) expression is key for certain B cell functions.

    Area of Science:

    • Immunology
    • Cell Biology

    Background:

    • B cells are crucial for adaptive immunity, producing antibodies of different isotypes.
    • Understanding B cell class commitment is vital for immune response regulation.

    Purpose of the Study:

    • To investigate the extent of B cell class commitment.
    • To correlate surface isotype expression with secreted isotype by B cell progeny.

    Main Methods:

    • Separation of primary and secondary B cells based on surface isotypes.
    • Analysis using the T-dependent splenic focus assay under optimal stimulation.

    Main Results:

    • Mature primary B cells predominantly express surface mu (IgM).
    • Immature B cells secrete IgA concurrently with IgM, not IgG.

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  • A significant portion of memory B cells express surface mu (IgM).
  • Memory B cells lacking surface mu (IgM) cannot produce IgM.

    • Conclusions:

    • Primary B cells exhibit potential for multiple antibody class production.
    • Memory B cell function is influenced by surface isotype expression.
    • B cell class commitment is dynamic and context-dependent.