Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

The detection of circulating immune complexes in normal subjects using four different methods

U Di Mario, K Guy, W J Irvine

    Journal of Clinical & Laboratory Immunology
    |March 1, 1981
    PubMed
    Summary

    This study compared four methods for detecting circulating immune complexes in blood donors. Immune complex levels varied by detection method and increased with age, but not between sexes.

    Related Concept Videos

    You might also read

    Related Articles

    Articles linked to this work by shared authors, journal, and citation graph.

    Sort by
    Same author

    Pillars Article: Antibodies to Pancreatic Islet Cells in Insulin-dependent Diabetics with Coexistent Autoimmune Disease. <i>Lancet</i>. 1974. 304: 1529-1531.

    Journal of immunology (Baltimore, Md. : 1950)·2017
    Same author

    Retrospective assessment of the validity and use of the community balance and mobility scale among individuals with subacute spinal cord injury.

    Spinal cord·2016
    Same author

    An ordered sequence of expression of human MHC class-II antigens during B-cell maturation?

    Immunology today·2014
    Same author

    Further intricacy of HLA-DR antigens.

    Immunology today·2014
    Same author

    Biobased plastics in a bioeconomy.

    Trends in biotechnology·2013
    Same author

    Bioplastics science from a policy vantage point.

    New biotechnology·2012

    Area of Science:

    • Immunology
    • Clinical Chemistry

    Background:

    • Circulating immune complexes (CICs) are implicated in various diseases.
    • Accurate detection of CICs is crucial for diagnosis and monitoring.
    • Multiple assays exist, necessitating comparison for reliable clinical application.

    Purpose of the Study:

    • To compare the performance of four distinct methods for quantifying CICs in human sera.
    • To evaluate the influence of demographic factors (age, sex) and temporal variations on CIC levels.

    Main Methods:

    • Solid phase C1q binding test
    • Fluid phase C1q binding assay
    • Conglutinin binding test
    • Raji cell radio-immune assay
    • Analysis of 336 blood donor sera, with 68-105 subjects per method.

    Related Experiment Videos

    Main Results:

    • Three methods (C1q binding, conglutinin) showed asymmetric distribution, while the Raji cell assay yielded wider spread.
    • No significant difference in CIC levels was observed between males and females.
    • A positive correlation between age and CIC prevalence was noted for C3-binding assays.
    • Intra-individual variations in CIC levels were detected over 24 hours, lacking a consistent pattern.

    Conclusions:

    • Method choice significantly impacts the distribution and prevalence of detected CICs.
    • Age is a relevant factor when interpreting CIC levels, particularly with C3-binding assays.
    • For small patient groups, serial sampling is recommended; for large cohorts, age-matched controls are essential for comparison.