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Characterization of a Pasteurella multocida bacteriophage

J L Gadberry, N G Miller

    American Journal of Veterinary Research
    |September 1, 1978
    PubMed
    Summary
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    The Pasteurella multocida bacteriophage B939a is a stable, temperate phage. Despite UV irradiation attempts, it did not produce lytic phage, but showed heat tolerance and a distinct morphology.

    Area of Science:

    • Microbiology
    • Virology
    • Bacteriology

    Background:

    • Pasteurella multocida is a significant bacterial pathogen.
    • Bacteriophages are viruses that infect bacteria and can be temperate or lytic.
    • Understanding phage characteristics is crucial for potential applications.

    Purpose of the Study:

    • To characterize the Pasteurella multocida bacteriophage B939a.
    • To determine the phage's stability and morphology.
    • To investigate its lytic potential.

    Main Methods:

    • Observation of plaque morphology (turbid plaques).
    • Attempted induction of lytic phage production via UV irradiation.
    • Assessment of phage stability at various temperatures (4°C, room temperature, 60°C, 70°C).

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  • Morphological analysis using electron microscopy (implied by description).

    • Main Results:

    • Bacteriophage B939a produced turbid plaques, indicative of a temperate phage.
    • UV irradiation failed to induce lytic phage production.
    • The phage demonstrated high stability at 4°C and relative stability at room temperature.
    • It tolerated moist heat at 60°C but was inactivated at 70°C within 10 minutes.
    • Morphologically, it was identified as a tailed phage with a hexagonal head.

    Conclusions:

    • Bacteriophage B939a is a stable, temperate phage of Pasteurella multocida.
    • Its resistance to UV-induced lysis and specific thermal inactivation points are key characteristics.
    • The tailed, hexagonal head morphology is a defining feature.