Related Experiment Video

Updated: Aug 11, 2026

Characterization of Thymus-dependent and Thymus-independent Immunoglobulin Isotype Responses in Mice Using Enzyme-linked Immunosorbent Assay
06:15

Characterization of Thymus-dependent and Thymus-independent Immunoglobulin Isotype Responses in Mice Using Enzyme-linked Immunosorbent Assay

Published on: September 7, 2018

Binding of peanut lectin to thymic cortex and germinal centres of lymphoid tissue

M L Rose, F Malchiodi

    Immunology
    |April 1, 1981
    PubMed

    The binding pattern of horseradish peroxidase conjugated peanut lectin (HRP-PNL) on frozen sections of lymphoid tissue from man, mouse, rat, hamster, guinea-pig, rabbit, sheep and chicken has been investigated. Binding of PNL was found to be highly species dependent; man, mouse and sheep showed strong binding to lymphocytes in thymic cortex and germinal centres; lymphoid tissue from hamster, guinea-pig and rabbit did not stain with HRP-PNL and rat showed only lightly positive cells in thymic cortex and germinal centres; all lymphoid tissue from chicken, except the bursal cortex, bound PNL. Neuraminidase treatment of tissues which did not bind PNL resulted in strongly PNL-positive cells. Double binding studies on murine Peyer's patches with fluorescein isothiocyanate conjugated PNL (FITC-PNL) and tetramethylrhodamine isothiocyanate (TRITC)-anti-Thy-1.2 or anti-immunoglobulin reagents revealed 3%-10% of PNL positive cells to be Thy-1.2 positive and 70%-80% to bear surface immunoglobulin.

    More Related Videos

    Antigenic Liposomes for Generation of Disease-specific Antibodies10:31

    Antigenic Liposomes for Generation of Disease-specific Antibodies

    Published on: October 25, 2018

    Unraveling Key Players of Humoral Immunity: Advanced and Optimized Lymphocyte Isolation Protocol from Murine Peyer's Patches08:25

    Unraveling Key Players of Humoral Immunity: Advanced and Optimized Lymphocyte Isolation Protocol from Murine Peyer's Patches

    Published on: November 21, 2018

    Related Concept Videos

    Lymphoid Cells and Tissues01:18

    Lymphoid Cells and Tissues

    Lymphoid cells and tissues are integral to the immune system, which is crucial in maintaining our body's defense against harmful pathogens. They form the building blocks of lymphoid organs, which include the spleen, thymus, and lymph nodes.
    Lymphoid cells consist of various types of immune system cells. These include B and T lymphocytes, which are responsible for producing antibodies and killing infected cells, respectively. Dendritic cells act as messengers between the innate and adaptive...
    Primary Lymphoid Organs01:16

    Primary Lymphoid Organs

    Primary lymphoid organs are pivotal in the formation, development, and maturation of lymphocytes, the white blood cells that serve as the backbone of our immune system. This crucial function underscores their fundamental role in maintaining our overall health and immunity. The two primary lymphoid organs of prime importance are the red bone marrow and the thymus.
    The red bone marrow is a soft, spongy tissue nestled in the interior of long bones such as the humerus and femur. It is the site...
    Secondary Lymphoid Organs01:15

    Secondary Lymphoid Organs

    Secondary organs, including lymph nodes, the spleen, and mucosa-associated lymphoid tissue (MALT), work harmoniously to protect us from disease and infection.
    The spleen is a vital organ in the lymphatic system, nestled in the upper left side of the abdomen. It is composed of two primary regions: the red pulp and the white pulp, each having distinct functions. The red pulp performs a significant role in blood filtration. It efficiently purges the blood of old or damaged red blood cells and...
    Cells of the Adaptive Immune Response01:23

    Cells of the Adaptive Immune Response

    The T and B lymphocytes of the adaptive immune system develop from common lymphoid progenitor cells in the bone marrow. These progenitors give rise to precursors that eventually develop into both T and B lymphocytes. As these precursors mature, they gain the ability to detect and respond to foreign antigens in the body, a process known as immunocompetence. Additionally, these precursors acquire self-tolerance, a process that ensures they do not react to self-antigens. This intricate system...
    T Cell Activation and Clonal Selection01:22

    T Cell Activation and Clonal Selection

    T cells are integral to our adaptive immune system, recognizing and effectively responding to foreign antigens. T cell activation and clonal selection are pivotal in orchestrating this immune response. This article elucidates these mechanisms, detailing the roles of cluster of differentiation (CD) markers, major histocompatibility complex (MHC) molecules, costimulatory signals, and the process of clonal selection.
    Naive T cells that have not yet encountered an antigen express two primary CD...
    B Cell Activation and Differentiation01:24

    B Cell Activation and Differentiation

    The adaptive immune response, a sophisticated defense mechanism, relies on the activation and differentiation of B lymphocytes, or B cells. These processes enable our bodies to mount a tailored response against specific pathogens such as bacteria, free virus particles, toxins, and parasites.
    When naive B cells encounter a specific antigen that can bind to the B cell receptor (BCR) on their surface, they undergo sensitization to respond to the antigen's presence. Sensitization begins with...