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Identification of the Gross cell surface antigen associated with murine leukemia virus-infected cells
Journal of Virology
|August 1, 1977
Abstract:
The Gross cell surface antigen (GCSA) is produced by cells that are either exogenously infected with murine leukemia virus (MuLV) or are expressing endogenous MuLV genomes. In immune precipitation assays, GCSA was resolved into two serologically distinct 85,000- and 95,000-dalton viral proteins. These antigenic components are glycosylated forms of the polyprotein precursors of the MuLV internal structural proteins.
Insights
Gross cell surface antigen (GCSA) involves two distinct viral proteins, 85-kDa and 95-kDa. These proteins are glycosylated precursors to murine leukemia virus (MuLV) internal structural proteins.
Area of Science:
- Virology
- Immunology
- Molecular Biology
Background:
- Murine leukemia virus (MuLV) infection or expression can lead to the production of specific cell surface antigens.
- Understanding these antigens is crucial for studying viral pathogenesis and host immune responses.
Purpose of the Study:
- To characterize the molecular nature of the Gross cell surface antigen (GCSA).
- To identify the viral proteins associated with GCSA expression.
Main Methods:
- Immune precipitation assays were utilized to isolate and analyze GCSA.
- Serological methods were employed to distinguish different antigenic components.
Main Results:
- GCSA was resolved into two distinct viral proteins with molecular masses of 85,000 and 95,000 daltons.
- These proteins were identified as glycosylated forms of polyprotein precursors.
Conclusions:
- The identified viral proteins are the antigenic components of GCSA.
- GCSA represents glycosylated precursors to MuLV internal structural proteins, providing insights into MuLV replication and assembly.