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Influence of Ara-C on effector activity of cytotoxic T-lymphocytes in vitro
Mouse T-lymphocytes carrying the H-2d haplotype were sensitized in vitro against allogeneic spleen cells homozygous for the H-2b haplotype. Cytolytic T-lymphocytes (CTL) were tested in a 4 hr cytotoxicity assay against 51Cr-labeled H-2b RBL-5 lymphoma cells. The cytotoxic activity of CTL was markedly increased by preincubation with Ara-C (1 through 1000 micrograms/ml, 1 hr at 37 degrees C). Limited but significant increase of cytotoxicity occurred when the drug was added to the effector-target mixture, or when target cells were preincubated with Ara-C using the same conditions adopted for the in vitro treatment of CTL. It is suggested that the present findings would contribute to elucidate the mechanism underlying antitumor synergistic effects between host's responses and treatment with antineoplastic agents.
Mouse T-lymphocytes carrying the H-2d haplotype were sensitized in vitro against allogeneic spleen cells homozygous for the H-2b haplotype. Cytolytic T-lymphocytes (CTL) were tested in a 4 hr cytotoxicity assay against 51Cr-labeled H-2b RBL-5 lymphoma cells. The cytotoxic activity of CTL was markedly increased by preincubation with Ara-C (1 through 1000 micrograms/ml, 1 hr at 37 degrees C). Limited but significant increase of cytotoxicity occurred when the drug was added to the effector-target mixture, or when target cells were preincubated with Ara-C using the same conditions adopted for the in vitro treatment of CTL. It is suggested that the present findings would contribute to elucidate the mechanism underlying antitumor synergistic effects between host's responses and treatment with antineoplastic agents.