[Chromatographic method on a thin sorbent layer for studying the purity and quality of the antitumor antibiotic,...

A method for qualitative and quantitative investigation of rubomycin composition was developed. It was based on chromatographic separation of the antibiotic components in a thin layer of silicic acid followed by their elution and spectrophotometric determination. An additional component of rubomycin found in the therapeutic preparations and the conditions of its formation were studied. Fifteen different lots of rubomycin were assayed and the requirements for the limits of the admixtures in the antibiotic therapeutic preparations were determined.