Pretrigeminal nucleus of leopard frog concentrates 2-deoxy-d-glucose during release calling

After injection of 14C-2-deoxy-D-glucose, some leopard frogs were induced to release call repeatedly, while others were used as silent controls. Autoradiograms of brains of many of the calling frogs, but none of the silent ones, showed heavy labeling of the pretrigeminal nucleus. This supports the hypothesis that this nucleus is involved in release calling.