Creating a useful panel of anti-T cell monoclonal antibodies

Monoclonal antibodies to human T lymphocyte surface markers were produced by cell fusions between splenocytes from mice, immunized with T lineage cells, and mouse myeloma cells. Our approaches to immunization, clone selection and analysis of the resultant monoclonal antibodies with similar reactivities were produced. In one example, we found that several distinct monoclonal antibodies identified the same T inducer subset yet, apparently, recognized epitopes of the differentiation antigen(s) on these cells.