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Increased ascorbic acid content in chronic lymphocytic leukemia B lymphocytes

L Liebes, R Krigel, S Kuo

    Proceedings of the National Academy of Sciences of the United States of America
    |October 1, 1981
    PubMed
    Summary

    Chronic lymphocytic leukemia (CLL) lymphocytes contain significantly higher levels of ascorbic acid compared to normal lymphocytes. This difference is primarily observed in CLL B cells, suggesting a potential role for ascorbic acid in the disease.

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    Area of Science:

    • Biochemistry
    • Hematology
    • Cell Biology

    Background:

    • Ascorbic acid (Vitamin C) is a vital antioxidant and essential nutrient.
    • Previous studies have not extensively investigated ascorbic acid levels in chronic lymphocytic leukemia (CLL) cells.

    Purpose of the Study:

    • To quantify and compare ascorbic acid levels in lymphocytes from healthy individuals and patients with CLL.
    • To determine if specific lymphocyte subtypes (B and T cells) contribute to observed differences in ascorbic acid content.

    Main Methods:

    • High-performance liquid chromatography (HPLC) was used to identify and quantify ascorbic acid in lymphocyte extracts.
    • Lymphocytes were isolated from the blood of normal subjects and untreated CLL patients.
    • Cellular enrichment techniques were employed to isolate and analyze B and T cell populations.

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    Main Results:

    • CLL lymphocytes exhibited significantly higher ascorbic acid concentrations (111 +/- 15.3 nmol/10^8 cells) compared to normal lymphocytes (42.2 +/- 3.3 nmol/10^8 cells).
    • The elevated ascorbic acid levels were predominantly found in CLL B cells, showing a 5- to 15-fold increase over normal B cells.
    • Ascorbic acid levels in T cells were comparable between normal and CLL subjects.

    Conclusions:

    • CLL B cells possess a markedly elevated concentration of ascorbic acid.
    • This high ascorbic acid content may provide CLL B cells with enhanced reducing capacity to counteract oxidative stress or free radicals.