Related Experiment Videos
Lymphokine production by peripheral blood leucocytes: quantitation of T-cell growth factor activity for assessment of
Australian and New Zealand Journal of Medicine
|October 1, 1981
Abstract:
Mitogen stimulation of as few as 200,000 human peripheral blood leucocytes (PBL) in 0.2 ml volumes produces highly active lymphokine supernatants. The lymphokine activity measured is T-cell growth factor. Under optimum conditions of PHA-P concentration, serum source and concentration, and time, activity is detectable to a dilution of between 1/250 and 1/700 for lymphokine supernatants from PBL of normal donors. Lymphokine supernatants prepared from PBL of donors previously treated with the immunosuppressive regimes of radiotherapy and/or chemotherapy had activities that were between 13% and 66% of normal controls. Lymphokine production as a quantitative measure of immune capability is discussed.