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Related Experiment Videos

Surface potentials and sodium entry in frog skin epithelium

D Benos, R Latorre, J Reyes

    The Journal of Physiology
    |December 1, 1981
    PubMed
    Summary

    Surface potential alterations minimally impact sodium (Na) entry across frog skin. Uranyl ions affect Na transport differently in various frog species, suggesting Na entry is independent of membrane surface potential changes.

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    Area of Science:

    • Physiology
    • Membrane Biophysics
    • Ion Transport

    Background:

    • The apical membrane's surface potential influences ion transport.
    • Understanding sodium (Na) entry mechanisms is crucial for epithelial physiology.

    Purpose of the Study:

    • To investigate the impact of apical membrane surface potential on Na entry in isolated frog skin.
    • To determine if changes in surface potential affect Na transport rates.

    Main Methods:

    • Examined Na entry into isolated Rana catesbeiana skin.
    • Assessed effects of altered external ionic strength.
    • Utilized uranyl ion (UO2(2+)) to induce surface potential changes in lipid monolayers and frog skin.
    • Measured short-circuit current (Isc) with and without amiloride.

    Main Results:

    • External ionic strength changes had minimal effect on Na transport.
    • Uranyl ion induced significant surface potential changes in lipid monolayers.
    • Uranyl ion modulated short-circuit current (Isc) in a species-dependent manner (inhibition in R. catesbeiana, stimulation in R. temporaria).
    • Amiloride blocked uranyl ion's effects on Isc, indicating involvement of epithelial sodium channels (ENaC).

    Conclusions:

    • The surface charge density near Na-selective entry sites is low.
    • Na entry is not significantly affected by changes in membrane surface potential.
    • Uranyl ion's effects on Isc are likely mediated by interactions with amiloride-sensitive Na+ channels.

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