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Related Experiment Videos

Decrease in stiffness during shortening in calcium activated skinned muscle fibers

T Tsuchiya, K Güth, H J Kuhn

    Pflugers Archiv : European Journal of Physiology
    |February 1, 1982
    PubMed
    Summary

    Muscle fiber shortening reduces force and stiffness. Faster shortening decreases tension and stiffness, indicating fewer attached crossbridges and less force per crossbridge in frog muscle fibers.

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    Area of Science:

    • Muscle physiology
    • Skeletal muscle mechanics
    • Biophysics

    Background:

    • Muscle contraction involves the interaction of actin and myosin filaments.
    • Crossbridge cycling is fundamental to force generation in muscle.
    • Understanding the dynamics of crossbridge attachment and detachment is crucial for muscle function.

    Purpose of the Study:

    • To investigate the mechanical behavior of single frog muscle fibers during ramp shortening.
    • To determine how force and stiffness change with varying speeds of shortening.
    • To elucidate the relationship between crossbridge dynamics and mechanical output.

    Main Methods:

    • Single frog muscle fibers (sartorius or semitendinosus) were mechanically skinned.
    • Fibers were activated in an ATP salt solution with 10 micro M Ca2+ at 7 degrees C.

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  • Isometric contractions were followed by ramp shortening at speeds from 0.03 to 2.4 s-1.
  • Fiber stiffness was measured using superimposed quick stretches during shortening.

    • Main Results:

    • Both force and stiffness decreased during ramp shortening, reaching a steady state around 60 ms.
    • Increased shortening speed led to decreased fiber tension and stiffness.
    • The ratio of stiffness to tension increased with faster shortening speeds.

    Conclusions:

    • Shortening in muscle fibers reduces both the number of attached crossbridges and the average force per crossbridge.
    • The rate of shortening significantly influences the mechanical properties of muscle fibers.
    • These findings provide insights into the kinetics of crossbridge interactions during muscle activity.