Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Primary structure of monkey osteocalcin

P V Hauschka, S A Carr, K Biemann

    Biochemistry
    |February 16, 1982
    PubMed
    Summary
    This summary is machine-generated.

    The amino acid sequence of osteocalcin in Macaca fascicularis was determined. Key features for calcium binding in this vitamin K-dependent bone protein are evolutionarily conserved.

    Related Concept Videos

    You might also read

    Related Articles

    Articles linked to this work by shared authors, journal, and citation graph.

    Sort by
    Same author

    Analysis of the kinetics and yields of OH radical production from the CH3OCH2 + O2 reaction in the temperature range 195-650 K: an experimental and computational study.

    The journal of physical chemistry. A·2014
    Same author

    Photon-induced dissociation with a four-sector tandem mass spectrometer.

    Journal of the American Society for Mass Spectrometry·2013
    Same author

    A computer program (COMPOST) for predicting mass spectrometric information from known amino acid sequences.

    Journal of the American Society for Mass Spectrometry·2013
    Same author

    Utility of N-peracetylation of proteins for their structure determination by mass spectrometry.

    Journal of the American Society for Mass Spectrometry·2013
    Same author

    Matrix-assisted laser desorption ionization mass spectrometry with 2-(4-hydroxyphenylazo)benzoic acid matrix.

    Journal of the American Society for Mass Spectrometry·2013
    Same author

    Selective detection of phosphopeptides in complex mixtures by electrospray liquid chromatography/mass spectrometry.

    Journal of the American Society for Mass Spectrometry·2013
    Same journal

    Aromatic Cage-Directed Azide-Methyllysine Photochemistry for Profiling Nonhistone Interacting Partners of the MeCP2 Methyl-CpG-Binding Domain.

    Biochemistry·2026
    Same journal

    Differential Hydroxypyruvate Processing by <i>E. coli</i> and <i>P. aeruginosa</i> DXP Synthases Reveals Preferential Xylulose 5-Phosphate Formation by the <i>P. aeruginosa</i> Enzyme.

    Biochemistry·2026
    Same journal

    Structural and Functional Characterization of Heterologous Nitrogenase Complexes.

    Biochemistry·2026
    Same journal

    Discovery of Bacterial Unspecific Peroxygenases.

    Biochemistry·2026
    Same journal

    Lactate Biology: Subcellular Routing and Chemical Form Define Function.

    Biochemistry·2026
    Same journal

    Nature's Anaerobic Toolkit: Glycyl Radical Enzymes and Their Expanding Functional and Mechanistic Diversity.

    Biochemistry·2026
    See all related articles

    Area of Science:

    • Biochemistry
    • Molecular Biology
    • Evolutionary Biology

    Background:

    • Osteocalcin is a crucial vitamin K-dependent protein found in bone matrix.
    • Understanding osteocalcin's structure is vital for comprehending bone metabolism and health.
    • Sequence analysis provides insights into protein function and evolutionary conservation.

    Purpose of the Study:

    • To determine the complete 49-residue amino acid sequence of osteocalcin from the old world monkey Macaca fascicularis.
    • To identify key structural features, including gamma-carboxyglutamic acid residues and disulfide bonds.
    • To investigate the evolutionary conservation of calcium-binding sites within osteocalcin.

    Main Methods:

    • Utilized a combination of gas chromatography-mass spectrometry (GC-MS) and Edman degradation techniques for efficient sequence determination.

    Related Experiment Videos

  • Applied established biochemical methods for protein sequencing and characterization.
  • Employed comparative sequence analysis to assess evolutionary conservation.

    • Main Results:

    • The complete 49-amino acid sequence of Macaca fascicularis osteocalcin was elucidated.
    • Identified three gamma-carboxyglutamic acid residues at positions 17, 21, and 24.
    • Confirmed the presence of a disulfide-bonded loop between residues 23 and 29.
    • Observed strong evolutionary conservation of features essential for Ca2+ binding.

    Conclusions:

    • The determined sequence provides a detailed molecular blueprint of Macaca fascicularis osteocalcin.
    • The conserved calcium-binding features suggest a fundamental role in bone function across species.
    • This research contributes to the understanding of osteocalcin's structure-function relationship and its evolutionary trajectory.