Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Ticarcillin bioassay

R M Bannatyne, R Cheung

    Antimicrobial Agents and Chemotherapy
    |October 1, 1981
    PubMed
    Summary
    This summary is machine-generated.

    A new bioassay accurately measures ticarcillin levels using Beneckea natriegens. This simple method provides clear results quickly, aiding in antibiotic monitoring.

    Related Concept Videos

    You might also read

    Related Articles

    Articles linked to this work by shared authors, journal, and citation graph.

    Sort by
    Same author

    Neutron imaging of the deuterium-tritium tamping gas volume in an inertial confinement fusion hohlraum.

    The Review of scientific instruments·2024
    Same author

    A qualitative study of labour pain relief information: what women get, what they want, and how it influences their decisions and satisfaction.

    International journal of obstetric anesthesia·2022
    Same author

    Prevalence, characteristics and mortality outcomes of obese, nonobese and lean NAFLD in the United States, 1999-2016.

    Journal of internal medicine·2020
    Same author

    Ethnic influence on nonalcoholic fatty liver disease prevalence and lack of disease awareness in the United States, 2011-2016.

    Journal of internal medicine·2020
    Same author

    Decreased tibial nerve movement in patients with failed back surgery syndrome and persistent leg pain.

    European spine journal : official publication of the European Spine Society, the European Spinal Deformity Society, and the European Section of the Cervical Spine Research Society·2019
    Same author

    The 2014 Survey on Living with Chronic Diseases in Canada on Mood and Anxiety Disorders: a methodological overview.

    Health promotion and chronic disease prevention in Canada : research, policy and practice·2016
    Same journal

    <i>In vitro</i> antibacterial activity of gepotidacin in combination with other antimicrobial agents against <i>Neisseria gonorrhoeae</i> isolates.

    Antimicrobial agents and chemotherapy·2026
    Same journal

    Development of domain-specific probes of <i>Plasmodium falciparum</i> heat shock protein 70-1.

    Antimicrobial agents and chemotherapy·2026
    Same journal

    Addressing therapeutic options for KPC-3-producing ST307-<i>Klebsiella pneumoniae</i>: insights from <i>in vitro</i> evolution and mutant prevention strategies.

    Antimicrobial agents and chemotherapy·2026
    Same journal

    Indole-based hybrids target both asexual parasites and gametocytes of <i>Plasmodium falciparum</i> and synergize with lumefantrine.

    Antimicrobial agents and chemotherapy·2026
    Same journal

    Lineage-specific loss of the type VI secretion system in <i>Acinetobacter baumannii</i> ST19 is associated with reduced accessory genome content.

    Antimicrobial agents and chemotherapy·2026
    Same journal

    Genomic diversity and topical antimicrobial resistance in <i>Staphylococcus aureus</i> from clinical and carriage populations in the New York-New Jersey region.

    Antimicrobial agents and chemotherapy·2026
    See all related articles

    Area of Science:

    • Microbiology
    • Pharmacology
    • Analytical Chemistry

    Background:

    • Accurate quantification of antibiotics like ticarcillin is crucial for effective patient treatment.
    • Existing bioassays may lack sensitivity, speed, or simplicity for routine clinical use.
    • Development of novel bioassays is needed to improve antibiotic therapeutic drug monitoring.

    Purpose of the Study:

    • To describe a novel, accurate, and rapid plate diffusion bioassay for ticarcillin.
    • To validate the performance characteristics of the new bioassay.
    • To demonstrate the utility of the assay for serum sample analysis.

    Main Methods:

    • Utilized the fast-replicating bacterium Beneckea natriegens for enhanced sensitivity.
    • Employed a 4% salt agar medium to optimize assay conditions.

    Related Experiment Videos

  • Measured zones of inhibition for quantitative ticarcillin determination.

    • Main Results:

    • Achieved well-defined zones of inhibition within 3 hours.
    • Established a limit of sensitivity of approximately 5.0 µg/ml for ticarcillin.
    • Demonstrated the assay's simplicity and minimal sample requirement (40 µl serum for duplicates).

    Conclusions:

    • The described plate diffusion bioassay offers an accurate and efficient method for ticarcillin quantification.
    • The assay's speed and sensitivity make it suitable for clinical settings.
    • This bioassay provides a valuable tool for therapeutic drug monitoring of ticarcillin.