Lymphocyte nuclear refringence in two inbred strains of rat. Modifications of NRT during adjuvant arthritis

Nuclear refringence test (NRT) was studied in two inbred strains of rats: Lewis (LEW) and Wistar AG (WAG), the first develops a severe arthritis while the later only slight inflammation. Before adjuvant injection, a good NRT to ConA, PHA and Isoprinosine was observed in LEW but a poor one in WAG. After adjuvant injection a striking difference appears between the two strains concerning ConA response: in LEW the response is significantly lower on day 14, while in WAG the initial poor response is not modified. These data suggest that in WAG the suppressor T lymphocytes are already activated in vivo and are no longer responsive in vitro. The responses to PHA and Isoprinosine are parallel in both strains, the NRT response diminishes on day 14 in LEW and on day 21 in WAG.