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Antigenic types of various human lymphoblastoid cell interferons
Biken Journal
|June 1, 1982
Summary
Human lymphoblastoid cell lines produce different types of interferon (IFN). T-cell lines produce only fibroblast interferon (Hu IFN-beta), while B-cell lines produce leukocyte interferon (Hu IFN-alpha), fibroblast interferon, or both.
Area of Science:
- Immunology
- Virology
- Cell Biology
Background:
- Human virus-induced interferon (IFN) exists in two antigenic types: human leukocyte IFN (Hu IFN-alpha) and human fibroblast IFN (Hu IFN-beta).
- Understanding the production of these IFNs by different human cell lines is crucial for their therapeutic applications.
Purpose of the Study:
- To investigate the antigenic types of interferons produced by various human T, B, and non-T non-B lymphoblastoid cell lines.
- To determine if the cell type influences the production of specific IFN subtypes.
Main Methods:
- Induction of interferon production in human lymphoblastoid cell lines (T, B, and non-T non-B) using Sendai virus.
- Antigenic typing of the produced interferons to differentiate between Hu IFN-alpha and Hu IFN-beta.
Main Results:
- Human T lymphoblastoid cell lines exclusively produced Hu IFN-beta.
- Human B lymphoblastoid cell lines exhibited varied IFN production, yielding Hu IFN-alpha, Hu IFN-beta, or a mixture of both.
- Non-T non-B lymphoblastoid cell lines produced a combination of Hu IFN-alpha and Hu IFN-beta.
Conclusions:
- The antigenic type of interferon produced by human lymphoblastoid cells correlates with the characteristics of the producer cell line.
- Further research is needed to elucidate the specific factors governing the production of different IFN subtypes.