Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Antigen-specific human T-cell hybridomas with helper activity

E C DeFreitas, S Vella, A Linnenbach

    Proceedings of the National Academy of Sciences of the United States of America
    |November 1, 1982
    PubMed
    Summary

    Researchers created human T cell hybridomas specific for tetanus toxoid (TeT). These hybridomas effectively helped B cells produce anti-TeT antibodies, demonstrating their potential in immune response studies.

    Related Concept Videos

    You might also read

    Related Articles

    Articles linked to this work by shared authors, journal, and citation graph.

    Sort by
    Same author

    Upregulation of Meis1 and HoxA9 in acute lymphocytic leukemias with the t(4 : 11) abnormality.

    Oncogene·2001
    Same author

    Cloning and characterization of CLLD6, CLLD7, and CLLD8, novel candidate genes for leukemogenesis at chromosome 13q14, a region commonly deleted in B-cell chronic lymphocytic leukemia.

    Cancer research·2001
    Same author

    Akt phosphorylates and regulates the orphan nuclear receptor Nur77.

    Proceedings of the National Academy of Sciences of the United States of America·2001
    Same author

    Critical role of the HMGI(Y) proteins in adipocytic cell growth and differentiation.

    Molecular and cellular biology·2001
    Same author

    FHIT gene therapy prevents tumor development in Fhit-deficient mice.

    Proceedings of the National Academy of Sciences of the United States of America·2001
    Same author

    Effect of adenoviral transduction of the fragile histidine triad gene into esophageal cancer cells.

    Cancer research·2001

    Area of Science:

    • Immunology
    • Cell Biology

    Background:

    • T cell hybridomas are valuable tools for studying antigen recognition and immune responses.
    • Understanding T cell help for B cell antibody production is crucial for vaccine development and immunotherapy.

    Purpose of the Study:

    • To generate antigen-specific human T cell hybridomas.
    • To characterize the helper function of these hybridomas in B cell antibody production.

    Main Methods:

    • Fusion of Jurkat T cell lymphoma line with normal human T cells specific for tetanus toxoid (TeT).
    • Selection of hybridomas based on interleukin 2 production and binding to TeT-pulsed monocytes.
    • Assay of B cell helper activity by measuring anti-TeT antibody production in vitro.

    Main Results:

    Related Experiment Videos

    • Successfully produced human T cell hybridomas specific for tetanus toxoid.
    • Hybridomas exhibited antigen-specific interleukin 2 production.
    • Demonstrated potent helper activity for semiautologous B cells, leading to high levels of anti-TeT antibody production.

    Conclusions:

    • Antigen-specific T cell hybridomas can be generated using established fusion techniques.
    • These hybridomas serve as a powerful model for studying T cell-B cell interactions and humoral immunity.
    • The developed hybridomas show promise for research in adaptive immunity and antibody-based therapies.