[A microagar culture system for clonal growth of human T-lymphocytes]

A simple and reproducible micro-agar culture system for cloning human T lymphocytes has been described. The system consists of an agar layer and a liquid phase, which enables the easy addition of test substances and a prolonged proliferation. Further advantages of this system are a low incubation volume and a better morphological, cytochemical, immunological and electron-microscopical study of colonies obtained from the liquid phase.