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Related Experiment Videos

Plasmid-coded ampicillin resistance in Haemophilus ducreyi

J A Thomson, Y R Bilgeri

    Antimicrobial Agents and Chemotherapy
    |October 1, 1982
    PubMed
    Summary

    Seven ampicillin-resistant Haemophilus ducreyi isolates harbor plasmids, with most coding for beta-lactamase. Some plasmids were transferable, indicating potential for antibiotic resistance spread in this bacterium.

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    Area of Science:

    • Microbiology
    • Molecular Biology
    • Genetics

    Background:

    • Ampicillin resistance is a growing concern in Haemophilus ducreyi infections.
    • Previous studies identified ampicillin-resistant isolates of H. ducreyi.

    Purpose of the Study:

    • To investigate the genetic basis of ampicillin resistance in H. ducreyi.
    • To characterize the plasmids harbored by ampicillin-resistant H. ducreyi isolates.
    • To determine the transferability of these plasmids.

    Main Methods:

    • Plasmid DNA isolation and characterization from H. ducreyi isolates.
    • Conjugation experiments to assess plasmid transferability.
    • Detection of beta-lactamase activity associated with plasmids.

    Main Results:

    • Seven ampicillin-resistant H. ducreyi isolates harbored plasmids of varying sizes (3.95, 5.2, 5.8, and 6.4 megadaltons).
    • All plasmids, except the 5.8-megadalton one, were associated with beta-lactamase production.
    • The 6.4- and 5.2-megadalton plasmids were efficiently transferred to a streptomycin-resistant H. ducreyi mutant via conjugation.

    Conclusions:

    • Plasmids play a significant role in ampicillin resistance in H. ducreyi.
    • Beta-lactamase production is a key mechanism of resistance mediated by these plasmids.
    • The conjugal transferability of certain plasmids suggests a mechanism for the dissemination of antibiotic resistance within H. ducreyi populations.

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