Isolation and rapid identification of Haemophilus ducreyi

During a 2-month period, 62 strains of Haemophilus ducreyi were isolated from 168 genital lesions and 2 lymph node aspirates. Of these strains, 22 were found on both chocolate agar and fetal bovine serum agar supplemented with vancomycin, 29 were found only on chocolate agar, and 9 were found only on fetal bovine serum agar. Two additional strains were isolated on sheep blood agar. All of these isolates were correctly identified with the RapID NH system (Innovative Diagnostic Systems, Inc., Decatur, Ga.) a new identification kit that has a database for Haemophilus, Neisseria, and other genera that include fastidious gram-negative bacilli.