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Penicillin tolerance in nutritionally variant streptococci

Y Holloway, J Dankert

Antimicrobial Agents and Chemotherapy

|December 1, 1982

Summary

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Nutritionally variant streptococci showed low penicillin susceptibility initially. However, with penicillinase and staphylococcal streak testing, all strains demonstrated high minimal bactericidal concentrations, indicating penicillin tolerance.

Area of Science:

Microbiology
Bacterial Pathogenesis
Antimicrobial Susceptibility Testing

Background:

Nutritionally variant streptococci (NVS) are a group of bacteria that require specific nutrients for growth.
Penicillin is a common antibiotic used to treat bacterial infections.
Accurate determination of antimicrobial susceptibility is crucial for effective treatment.

Purpose of the Study:

To evaluate the susceptibility of nutritionally variant streptococci to penicillin.
To determine the true minimal bactericidal concentrations (MBCs) of penicillin against NVS.
To investigate potential mechanisms of penicillin tolerance in NVS.

Main Methods:

Broth dilution method was employed to determine minimal inhibitory concentrations (MICs).

Related Experiment Videos

MBCs were assessed using subculture methods, initially without and then with penicillinase and a staphylococcal streak.

Eleven distinct strains of nutritionally variant streptococci were analyzed.

Main Results:

All tested NVS strains exhibited low MICs to penicillin.
Standard subculture methods without penicillinase failed to reveal true MBCs.
In the presence of penicillinase and staphylococcal streak, all 11 NVS strains displayed high MBCs, confirming penicillin tolerance.

Conclusions:

Nutritionally variant streptococci can exhibit tolerance to penicillin, masking true resistance.
Standard susceptibility testing methods may underestimate penicillin's efficacy against NVS.
Modified testing protocols incorporating penicillinase are essential for accurate assessment of penicillin tolerance in NVS.