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Computerised acquisition and analysis of flow cytometric data

G Blackledge, R Swindell, B W Hodgson

    International Journal of Bio-Medical Computing
    |January 1, 1980
    PubMed
    Summary
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    A new computer interface for flow cytometry enables detailed cell analysis and data storage. This economical system optimizes data acquisition for advanced research without cell sorting capabilities.

    Area of Science:

    • Biotechnology
    • Computational Biology
    • Cell Biology

    Background:

    • Flow cytometry generates large datasets requiring efficient storage and analysis.
    • Integrating commercial flow cytometers with computers is crucial for modern biological research.

    Purpose of the Study:

    • To describe a novel computer interface for a commercial flow cytometer.
    • To develop programs for data acquisition and analysis.
    • To provide an economical and flexible solution for flow cytometry data management.

    Main Methods:

    • An interface was developed between an Ortho Cytofluorograf 4800 flow cytometer and an H.P. 9845S desktop computer.
    • Software was created for data acquisition, including cell DNA content analysis and 2-parameter (3D) histograms.
    • Sophisticated sub-population selection algorithms were implemented.

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    Main Results:

    • The system allows for optimized data acquisition per cell.
    • Detailed analysis of flow cytometry data, including sub-population identification, is achievable.
    • The interface provides a flexible and user-friendly data storage and analysis solution.

    Conclusions:

    • The developed system offers an economical and efficient method for analyzing flow cytometry data.
    • This interface enhances the capabilities of commercial flow cytometers for research applications.
    • The system facilitates advanced data analysis without requiring cell sorting functionality.