Genetic mapping of arg1 and arg8 in Saccharomyces cerevisiae by trisomic analysis combined with interallelic
Abstract:
Through use of multiply disomic strains, the genes arg1 and arg8 were excluded from all of chromosomes I to XVII except (i) XV and (ii) IX and XV, respectively. Further aneuploid analyses showed that these two genes were on the same chromosome. By tetrad analysis, arg1 was shown to be linked to SUP3 on the left arm of chromosome XV (parental ditype:nonparental ditype:tetratype = 74; 6:139) and arg8 was shown to be loosely linked to arg1 (parental ditype:nonparental ditype:tetratype 72:17:220) on the same arm. The sequence of the genes on this chromosome arm is centromere-SUP3-arg8. Because arg1 had previously been used to define an 18th chromosome, these results reestablished the minimum chromosome number in Saccharomyces cerevisiae as 17.
