Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Detection of human ADCC activity using automated flow cytometry

A M Attallah, P D Naguchi, T Folks

    Experientia
    |January 15, 1980
    PubMed
    Summary

    We developed a rapid assay to measure human antibody-dependent cell-mediated cytotoxicity (ADCC). This new method uses automated flow cytometry and detects significant killing in just one hour.

    Related Concept Videos

    You might also read

    Related Articles

    Articles linked to this work by shared authors, journal, and citation graph.

    Sort by
    Same author

    SDoH Impact on Periodontal Disease Using Machine Learning and Dental Records.

    Journal of dental research·2025
    Same author

    Diagnostic role of collagen-III and matrix metalloproteinase-1 for early detection of hepatocellular carcinoma.

    British journal of biomedical science·2020
    Same author

    A panel of a mitogenic (PDGF), biochemical (albumin) and demographic (age) parameters for the non-invasive assessment of hepatic fibrosis.

    British journal of biomedical science·2019
    Same author

    IL-28B rs12979860 polymorphism affect the course of chronic hepatitis and the development of HCC in Egyptian patients with hepatitis C type 4.

    British journal of biomedical science·2018
    Same author

    Clinical value of a diagnostic score for colon cancer based on serum CEA, CA19-9, cytokeratin-1 and mucin-1.

    British journal of biomedical science·2018
    Same author

    Fibro-Mark: a panel of laboratory parameters for predicting significant fibrosis in chronic hepatitis C patients.

    British journal of biomedical science·2017

    Area of Science:

    • Immunology
    • Cellular Biology
    • Assay Development

    Background:

    • Antibody-dependent cell-mediated cytotoxicity (ADCC) is a crucial immune mechanism.
    • Accurate and rapid measurement of ADCC is important for research and drug development.
    • Existing ADCC assays can be time-consuming or lack sensitivity.

    Purpose of the Study:

    • To develop a rapid and sensitive assay for measuring human antibody-dependent cell-mediated cytotoxicity (ADCC).
    • To utilize automated flow cytometry for efficient ADCC quantification.
    • To establish a new standard for ADCC assessment.

    Main Methods:

    • Automated flow cytometry was employed for high-throughput analysis.
    • Cell cultures were stained for DNA content to differentiate target and effector cells.
    • ADCC was quantified by measuring the relative changes in cell populations.

    Main Results:

    • The assay demonstrated high sensitivity, detecting 42% killing at a low effector to target ratio of 2:1.
    • Significant ADCC activity was measurable within a 1-hour incubation period.
    • The method allows for clear distinction between human effector cells and chick red blood cell targets.

    Conclusions:

    • The developed automated flow cytometry assay provides a rapid and sensitive method for measuring human ADCC.
    • This assay has the potential to accelerate immunological research and therapeutic antibody development.
    • The technique offers a robust platform for quantitative ADCC analysis.

    Related Experiment Videos