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Related Experiment Videos

B cell antibodies and crossmatching

G Ayoub, M S Park, P I Terasaki

    Transplantation
    |March 1, 1980
    PubMed
    Summary

    Positive B cell crossmatches impact kidney transplant survival. Cold antibodies may improve graft survival, while strong warm HLA-DR antibodies are detrimental to transplant success.

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    Clinical and experimental immunology·2017

    Area of Science:

    • Immunology
    • Transplantation Medicine
    • Histocompatibility

    Background:

    • Crossmatching is crucial for organ transplantation success.
    • B cell crossmatches identify potential antibody-mediated rejection.
    • Antibody characteristics (warm vs. cold) may influence outcomes.

    Purpose of the Study:

    • To evaluate the impact of B cell crossmatch results on 1-year kidney transplant survival.
    • To differentiate the effects of cold and warm antibodies in B cell crossmatches.
    • To assess the role of HLA-DR antibodies in B cell crossmatch-positive transplants.

    Main Methods:

    • Crossmatching of over 200 cadaver donor transplants against T and B lymphocytes.
    • Testing performed at 5°C (cold) and 37°C (warm) conditions.
    • Analysis of 1-year transplant survival rates based on crossmatch antibody type.

    Main Results:

    • 54 positive B cell crossmatches were identified.
    • 1-year survival was 58% for B-cold-positive and 42% for B-warm-positive crossmatches.
    • Strong HLA-DR antibodies in B-warm-positive crossmatches showed a significantly lower 1-year survival rate of 27%.

    Conclusions:

    • B cell crossmatches positive for HLA-DR antibodies are associated with poor transplant outcomes.
    • Cold anti-IgM antibodies in B cell crossmatches may be associated with enhanced graft survival.
    • Differentiating antibody type in B cell crossmatches is critical for predicting transplant success.

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