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Physiological evaluation of the isolated perfused rat kidney
The American Journal of Physiology
|February 1, 1980
Summary
The isolated perfused rat kidney is useful for studying glomerular and proximal tubule functions, though some renal hemodynamic and distal tubule abnormalities persist. A nonfiltering model aids in studying proximal tubule cell transport and metabolism.
Area of Science:
- Nephrology
- Renal Physiology
- Experimental Biology
Background:
- The isolated perfused rat kidney (IPRK) is a valuable model for studying kidney function.
- A thorough evaluation is needed to understand its limitations and applications in research.
Purpose of the Study:
- To critically assess the functional capabilities of the IPRK for renal function studies.
- To identify preserved and abnormal functions within the IPRK model.
Main Methods:
- Utilized clearance and micropuncture techniques in IPRK models.
- Perfused kidneys with a plasmalike medium containing albumin, glucose, and amino acids.
Main Results:
- Proximal convoluted tubule reabsorption of organic substances, electrolytes, and fluid was well-preserved.
- Glomerular filtration rate and permselectivity were maintained.
- Abnormalities were observed in renal hemodynamics, urinary concentration-dilution, and distal nephron function.
Conclusions:
- The IPRK is suitable for studying glomerular and proximal tubule functions.
- Renal hemodynamic and distal nephron abnormalities may be attributed to perfusate viscosity and altered distal tubule function.
- A nonfiltering IPRK model allows independent study of proximal tubular cell transport and metabolism.
