Prospects for large scale production of thermolabile products using anchorage dependent cells

Availability of culture systems for the large scale production of anchorage dependent cells is briefly outlined. Studies on two thermolabile viruses (Measles and Influenza), showing inactivation rates of 0.1 - 0.2 log 10 per hour, clearly demonstrate the need for minimal residence time in the culture system. Logistical considerations indicate that small capacity perfusion culture systems for anchorage dependent cells would give increased recovery of these products and may readily be combined with subsequent processing steps.