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A simple reliable procedure for obtaining metaphases from human leukemic bone-marrow aspirates suitable for Giemsa
In Vitro
|February 1, 1980
Abstract:
Short incubation of heparinized human leukemic bone-marrow cells in phosphate buffered saline containing colcemid and overnight chilling of fixed cells yields metaphases with elongated and well-spread chromosomes. This technique enables us to do trypsin-Giemsa banding of chromosomes obtained from leukemic marrow cells otherwise difficult to band.