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Murine cell surface immunoglobulin: two native IgD structures

R R Pollock, M F Mescher

    Journal of Immunology (Baltimore, Md. : 1950)
    |April 1, 1980
    PubMed
    Summary
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    Murine spleen cells express two native forms of immunoglobulin D (IgD) on their surface, IgDI and IgDII. Their relative amounts vary significantly between different mouse strains.

    Area of Science:

    • Immunology
    • Molecular Biology
    • Biochemistry

    Background:

    • Immunoglobulin D (IgD) is a key surface receptor on mature B lymphocytes.
    • The structural heterogeneity of IgD on cell surfaces has been previously debated.

    Purpose of the Study:

    • To characterize the distinct molecular forms of murine spleen cell surface IgD.
    • To investigate the native structural integrity of these IgD forms.

    Main Methods:

    • Sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) under nonreducing conditions.
    • Analysis of molecular weight and potential degradation or rearrangement products.

    Main Results:

    • Two distinct forms of IgD, designated IgDI and IgDII, were identified on intact murine spleen cells.

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  • IgDII exhibited an apparent molecular weight of 96,000 Da, consistent with an H L structure.
  • IgDI showed an apparent molecular weight of 150,000 Da, suggesting a modified H2L2 structure.
  • Neither form resulted from proteolytic degradation or disulfide bond rearrangement.
  • Significant strain-dependent variations in the relative proportions of IgDI and IgDII were observed.

    • Conclusions:

    • Murine spleen cells express at least two native, distinct molecular forms of surface IgD.
    • The observed structural differences and strain variations highlight the complexity of IgD expression and function.