[Determination of the effectiveness of Chinese hamster cell fusion in monolayers]

Using polyethylene glycol 6000 (PEG), cell fusion was obtained for the Chinese hamster transplantable culture. A most rational character of cell fusion efficiency was the amount of nuclei found in multinuclear cells (polykaryocytar index) determined within 30-60 minutes after PEG treatment of the culture. After a 1-6 hour treatment, a mass death of polykaryocytes was more than 8 hours; in 3 hours and up to the end of a 24 hour's period of observation an intensive cell division is observed.