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Demonstration of a complement-dependent migration inhibitory activity in normal guinea pig serum
Allergy
|January 1, 1980
Summary
Normal guinea pig serum (GPS) inhibits cell migration, particularly granulocytes. This effect relies on complement factors, as it
Area of Science:
- Immunology
- Cell Biology
Background:
- Normal guinea pig serum (GPS) exhibits cell migration inhibitory effects.
- Granulocytes are more sensitive to this inhibition than lymphomonocytes or macrophages.
Purpose of the Study:
- To investigate the role of complement in the cell migration inhibitory activity of GPS.
- To identify the components responsible for this inhibitory effect.
Main Methods:
- Assessing cell migration inhibition using granulocytes, lymphomonocytes, and macrophages.
- Utilizing complement-inactivating agents (heat, EDTA, heparin, zymosan, immune complexes).
- Fractionating GPS and testing for restored hemolytic and migration inhibitory functions.
Main Results:
- GPS's migration inhibitory activity is abrogated by complement-destroying agents.
- Dialysis fractions of GPS restored both hemolytic and inhibitory functions upon recombination.
- Absorption of GPS on blood cells removed inhibitory activity but retained hemolytic function.
Conclusions:
- The cell migration inhibitory activity of GPS is dependent on complement factors.
- Specific complement components are essential for mediating the observed inhibition of cell migration.