Demonstration of migration inhibitory factor (MIF) in a murine system using myelomonocytic leukemia cells (WEHI-3)

Cells of the myelomonocytic tumor cell line, WEHI-3, were used as indicator cells in the indirect capillary test for the detection of migration inhibitory factor (MIF). A migration inhibition of about 50% was found and the results were highly reproducible. The indicator cells can be obtained in large quantities, as the myelomonocytic cells grow as an ascitic tumor in the peritoneal cavity of BALB/c mice.