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Bacterial mutagenicity and mammalian cell DNA damage by several substituted anilines

D Zimmer, J Mazurek, G Petzold

    Mutation Research
    |April 1, 1980
    PubMed

    Insights

    This study assessed the mutagenicity of substituted anilines in bacteria and their DNA-damaging effects in mammalian cells, correlating these with carcinogenicity. Some compounds showed mutagenic and DNA-damaging potential, highlighting risks associated with certain aniline derivatives.

    Area of Science:

    • Toxicology
    • Genetics
    • Chemical Carcinogenesis

    Background:

    • Substituted anilines are widely used in industrial processes.
    • Some aniline derivatives are known or suspected carcinogens.
    • Understanding their genotoxic potential is crucial for risk assessment.

    Purpose of the Study:

    • To evaluate the mutagenic activity of various alkyl- and haloanilines in Salmonella typhimurium.
    • To assess the DNA-damaging capacity of these compounds in mammalian V79 cells.
    • To correlate genotoxicity data with reported carcinogenicity of these anilines.

    Main Methods:

    • Bacterial mutagenicity testing using Salmonella typhimurium (Ames test).
    • Mammalian cell DNA damage assay using V79 cells.
    • Correlation analysis between in vitro genotoxicity and in vivo carcinogenicity data.

    Main Results:

    • Four of nine suspected carcinogenic anilines were identified as bacterial mutagens.
    • Four of seven tested anilines demonstrated DNA-damaging effects in V79 cells.
    • Specific compounds like 2,4,5-trimethylaniline and 2-methyl-4-chloroaniline showed significant mutagenic and DNA-damaging properties.

    Conclusions:

    • Genotoxicity assays can predict carcinogenic potential for some substituted anilines.
    • Certain alkyl- and haloanilines possess mutagenic and DNA-damaging capabilities.
    • Findings contribute to the toxicological profiling and risk assessment of aniline derivatives.

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