Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

[Morphological changes in liver tissue in experimental porphyria]

A Chlumská, J Dobiás, V Janousek

    Ceskoslovenska Patologie
    |May 1, 1978
    PubMed
    Summary

    Gricine administration in mice causes observable liver tissue changes, including porphyrin accumulation in hepatocytes and bile ducts within 72 hours. These changes are linked to increased enzyme activity and distinct crystalline porphyrin structures.

    Related Concept Videos

    You might also read

    Related Articles

    Articles linked to this work by shared authors, journal, and citation graph.

    Sort by
    Same author

    Activity of the novel siderophore cephalosporin cefiderocol against multidrug-resistant Gram-negative pathogens.

    European journal of clinical microbiology & infectious diseases : official publication of the European Society of Clinical Microbiology·2017
    Same author

    Cross-resistance to human cationic antimicrobial peptides and to polymyxins mediated by the plasmid-encoded MCR-1?

    Clinical microbiology and infection : the official publication of the European Society of Clinical Microbiology and Infectious Diseases·2017
    Same author

    [Shape-memory osteosynthesis for trapeziometacarpal joint arthrodesis].

    Acta chirurgiae orthopaedicae et traumatologiae Cechoslovaca·2014
    Same author

    [Examination of lymph nodes in resected colon segments with colorectal carcinoma].

    Rozhledy v chirurgii : mesicnik Ceskoslovenske chirurgicke spolecnosti·2013
    Same author

    Silver release from silver nanoparticles in natural waters.

    Environmental science & technology·2013
    Same author

    Perineural cell differentiation in ganglioneuromas. Report of 8 cases with immunohistochemical expression of perineural cell markers.

    Ceskoslovenska patologie·2012

    Area of Science:

    • Hepatology
    • Toxicology
    • Biochemistry

    Context:

    • Investigating the toxicological effects of gricine on liver tissue.
    • Utilizing mouse models to study drug-induced liver injury.
    • Examining cellular and subcellular changes following xenobiotic exposure.

    Purpose:

    • To characterize the temporal and morphological changes in mouse liver following gricine administration.
    • To identify the specific cellular compartments affected by gricine.
    • To assess the biochemical alterations associated with gricine exposure.

    Summary:

    • Microscopic liver changes were observed 42 hours post-gricine administration, characterized by porphyrin presence in hepatocyte cytoplasm, leading to cellular enlargement.
    • By 72 hours, porphyrins were detected in bile duct lumina, accompanied by hepatocyte dispersion extinction and Kupffer cell pigment accumulation.
    • Histochemical analysis revealed increased alkaline phosphatase, acidic phosphatase, glucose-6-phosphate dehydrogenase (G6PDH), and tetrazolium reductase activities. Electron microscopy confirmed crystalline porphyrin structures with birefringence and red fluorescence.

    Impact:

    • Provides detailed insights into the hepatotoxic mechanisms of gricine.
    • Establishes a timeline for gricine-induced liver damage in a murine model.
    • Highlights the utility of histochemical and electron microscopy techniques in evaluating xenobiotic toxicity.

    Related Experiment Videos