Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Insulin degradation by mononuclear cells

A C Powers, S S Solomon, W C Duckworth

    Diabetes
    |January 1, 1980
    PubMed
    Summary
    This summary is machine-generated.

    Peripheral blood mononuclear cells, primarily monocytes, bind and degrade insulin. This activity mirrors insulin receptor status in target tissues, offering insights into metabolic diseases like obesity.

    Related Concept Videos

    You might also read

    Related Articles

    Articles linked to this work by shared authors, journal, and citation graph.

    Sort by
    Same author

    Diversity of hepatitis C virus infection among HIV-infected people who inject drugs in India.

    Virusdisease·2020
    Same author

    Lenalidomide as a novel therapy for gastrointestinal angiodysplasia in von Willebrand disease.

    Haemophilia : the official journal of the World Federation of Hemophilia·2018
    Same author

    Occult HBV infection in HIV-infected adults and evaluation of pooled NAT for HBV.

    Journal of viral hepatitis·2018
    Same author

    Directly observed therapy of sofosbuvir/ribavirin +/- peginterferon with minimal monitoring for the treatment of chronic hepatitis C in people with a history of drug use in Chennai, India (C-DOT).

    Journal of viral hepatitis·2017
    Same author

    Evaluation of dried blood spot as an alternative specimen for the diagnosis of anti-HCV in resource-limited settings.

    Indian journal of medical microbiology·2014
    Same author

    The diagnosis of insulitis in human type 1 diabetes.

    Diabetologia·2013

    Area of Science:

    • Biochemistry
    • Cell Biology
    • Metabolic Research

    Background:

    • Peripheral blood mononuclear cells (PBMCs) have insulin receptors, with numbers or binding affinity altered in metabolic diseases like obesity.
    • Monocytes, not lymphocytes, specifically bind insulin, reflecting receptor status in key tissues (liver, muscle, fat).
    • Insulin degradation occurs in liver, muscle, and fat, prompting investigation into PBMCs' insulin-degrading capacity.

    Purpose of the Study:

    • To investigate insulin-binding and degradation by peripheral blood mononuclear cells.
    • To determine if PBMCs can serve as a model for insulin receptor status and metabolism in target tissues.

    Main Methods:

    • Incubation of intact PBMCs with 125I-insulin, measuring degradation via trichloroacetic acid precipitation.

    Related Experiment Videos

  • Assessing insulin degradation and binding under varying cell concentrations, incubation times, and temperatures.
  • Examining insulin degradation in homogenized PBMCs and subcellular fractions.
  • Testing purified lymphocyte preparations for insulin binding and degradation.

    • Main Results:

    • Insulin-degrading activity and total insulin binding in PBMCs increased with cell number and incubation time.
    • Degradation activity showed temperature dependence, with minimal activity at 4°C or 15°C.
    • Homogenized PBMCs degraded 2-5 times more insulin than intact cells, with Michaelis constant (Km) values of 7.03 x 10⁻⁸ M (intact) and 2.2 x 10⁻⁸ M (homogenized).
    • Significant degrading activity was found in the 100,000 x g supernatant of PBMCs; lymphocytes showed minimal binding and degradation.

    Conclusions:

    • Peripheral blood monocytes possess specific insulin-binding and degrading capabilities.
    • PBMC insulin binding and degradation may reflect insulin receptor status and metabolic activity in major insulin target tissues.
    • Monocytes represent a viable model for studying insulin interactions in peripheral blood, relevant to metabolic disease research.