Isolation and characterization of arginine auxotrophs of Cryptococcus neoformans

Arginine auxotrophs were isolated in both mating types of Cryptococcus neoformans. In both mutants, the auxotrophy was due to the lack of active argininosuccinate lyase. The virulence in mice of the mutants was compared with that of the wild type. One auxotroph displayed a loss of virulence which appeared to be related to the presence of another mutation, one which prevented normal cell separation after budding. The other auxotroph had reduced virulence compared with the wild type, but a variant isolated from it by mouse passage had virulence equivalent to that of the wild type while maintaining the auxotrophic requirements.