Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

A bactericidal activity of microsomal preparations

M Anders, E C McCoy, H S Rosenkranz

    Mutation Research
    |May 1, 1980
    PubMed
    Summary
    This summary is machine-generated.

    Related Concept Videos

    You might also read

    Related Articles

    Articles linked to this work by shared authors, journal, and citation graph.

    Sort by
    Same author

    The Development and Characterisation of a Structure-activity Relationship Model of the Draize Eye Irritation Test.

    Alternatives to laboratory animals : ATLA·2015
    Same author

    The High Production Volume Chemical Challenge Program : The Rodent LD50 and its Possible Replacement.

    Alternatives to laboratory animals : ATLA·2014
    Same author

    Structural Basis of the Toxicity of Chemicals in Cultured Human HeLa Cells.

    Alternatives to laboratory animals : ATLA·2014
    Same author

    A battery of cell toxicity assays as predictors of eye irritation: a feasibility study.

    Alternatives to laboratory animals : ATLA·2014
    Same author

    Structure-activity approach to the identification of environmental estrogens: the MCASE approach.

    SAR and QSAR in environmental research·2004
    Same author

    A substructure-based SAR model for odor perception in humans relevant to health risk assessments.

    SAR and QSAR in environmental research·2003
    Same journal

    Monoallelic germline RAD51C, RAD51D, and BRIP1 variants in hereditary cancer testing: Variant spectrum and clinical counselling implications.

    Mutation research·2026
    Same journal

    Prediction of hepatocellular carcinoma associated biomarkers in TP53 gene; A comprehensive in silico analysis.

    Mutation research·2026
    Same journal

    IDH1 mutation promotes angiogenesis via upregulation of hypoxia inducible factor 1 alpha in glial tumors.

    Mutation research·2026
    Same journal

    Targeting overexpressed oncogenes in esophageal cancer through miRNA-mediated gene silencing: Insights from binding affinity and thermodynamic profiling.

    Mutation research·2026
    Same journal

    The active compound quercetin from Polygonum cuspidatum targets COL3A1 to enhance CD8⁺ T cell cytotoxicity in gastric cancer.

    Mutation research·2026
    Same journal

    E2F1 promotes LIHC malignant phenotype via NEK2-mediated Wnt/β-catenin and Notch activation and EMT.

    Mutation research·2026
    See all related articles

    Mouse liver extracts (S9) significantly reduced the viability of Salmonella typhimurium strains. This toxic effect was eliminated by heat treatment, though the specific toxic agent in S9 remains unidentified.

    Area of Science:

    • Toxicology
    • Microbiology
    • Biochemistry

    Background:

    • Bacterial viability assays are crucial for assessing environmental and biological toxic effects.
    • Mouse hepatic post-mitochondrial supernatants (S9) are commonly used in genotoxicity testing.
    • Salmonella typhimurium strains are standard bacterial models for mutagenicity studies.

    Purpose of the Study:

    • To investigate the effect of mouse hepatic post-mitochondrial supernatants (S9) on Salmonella typhimurium viability.
    • To characterize the nature of the observed lethal activity in S9 fractions.

    Main Methods:

    • Bacterial suspensions of Salmonella typhimurium strains (TA1535, TA1537, TA98) were prepared.
    • Exposure of bacterial suspensions to mouse hepatic post-mitochondrial supernatants (S9).

    Related Experiment Videos

  • Assessment of bacterial viability following S9 exposure.
  • Heat treatment of S9 fractions at 56°C for 30 minutes to determine thermal stability of the toxic component.

    • Main Results:

    • Exposure to mouse hepatic S9 resulted in a significant loss of viability in Salmonella typhimurium tester strains.
    • The lethal activity of the S9 fraction was abolished by heating at 56°C for 30 minutes.
    • The specific toxic component within the S9 fraction responsible for bacterial lethality was not identified.

    Conclusions:

    • Mouse hepatic S9 possesses a heat-labile toxic component that adversely affects Salmonella typhimurium viability.
    • Further investigation is required to identify the specific toxic agent in S9 responsible for this observed bacterial toxicity.
    • These findings highlight the need to consider potential S9-mediated bacterial toxicity in genotoxicity assays.