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Leukocyte migration in different systems. Effect of colchicin. Kinetics of migration under agarose
Abstract:
In a double-filter modification of Boyden's method, formylmethionylleucylphenylalanine (FMLP) needed the presence of fresh plasma to induce leukocyte locomotor kinetics which compared with a model for directional migration. FMLP, or zymosan-activated plasma, did not stimulate migration in capillary tubes. The kinetics of leukocyte migration under agarose towards zymosan-activated plasma were more compatible to a model for increased random motility than for directional migration. Treatment of leukocytes with colchicin reduced their migration under gradient and non-gradient conditions in millipore filters, capillary tubes, and in under-agarose experiments.
Insights
Formylmethionylleucylphenylalanine (FMLP) requires fresh plasma for directed leukocyte migration. However, FMLP and zymosan-activated plasma showed increased random motility rather than directional migration in other assays.
Area of Science:
- Immunology
- Cell Biology
Background:
- Leukocyte migration is crucial for immune responses.
- Formylmethionylleucylphenylalanine (FMLP) is a known chemoattractant.
- Understanding leukocyte kinetics informs immune cell function research.
Purpose of the Study:
- To investigate leukocyte locomotor kinetics induced by FMLP and zymosan-activated plasma.
- To compare migration patterns with models of directional and random motility.
- To assess the effect of colchicine on leukocyte migration.
Main Methods:
- Double-filter modification of Boyden's method.
- Capillary tube assays.
- Under-agarose migration experiments.
- Treatment with colchicine.
Main Results:
- FMLP required fresh plasma for directional migration kinetics.
- FMLP and zymosan-activated plasma did not stimulate migration in capillary tubes.
- Leukocyte migration under agarose suggested increased random motility.
- Colchicine reduced leukocyte migration across all tested methods.
Conclusions:
- Leukocyte migration responses are complex and assay-dependent.
- FMLP's role in directional migration is influenced by plasma factors.
- Colchicine significantly impairs leukocyte motility.