[Determination of the renin concentration in the rat brain using a heterologous substrate]

The author introduced a method for determination of brain renin concentration, using the method of Boucher for determination of renin concentration in the kidney and plasma, adapted for brain tissue by Ganten. He defined parameters of enzymic reaction, when the brain renin concentration of a rat was examined by the usage of a nonstandardized dog substrate, prepared by the author. For this purpose she carried out studies on the kinetics of the reaction brain renin of a rat--a dog angiotensinogen. She described the influence of the enzymic substrate concentration and time on the course of the reaction. Furthermore she indicated the preparation of angiotensinogen from a dog plasma, and pointed out its advantages in determining renin in the brain of a rat. The mean value of the brain renin concentration in the rats of the Wistar strain was 140 ng/gm/h.