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Glucose clamping using the Biostator GCIIS

C A Verdonk, R A Rizza, R E Westland

    Hormone and Metabolic Research = Hormon- Und Stoffwechselforschung = Hormones Et Metabolisme
    |April 1, 1980
    PubMed
    Summary

    This study demonstrates that the Biostator GCIIS can maintain stable glucose levels during hypoglycemic, euglycemic, and hyperglycemic clamps in normal subjects. Despite minor technical challenges, satisfactory glucose control was achieved for two hours.

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    Area of Science:

    • Endocrinology
    • Metabolic Research
    • Biomedical Engineering

    Background:

    • Accurate glucose control is crucial for metabolic research.
    • The Biostator GCIIS is a device for automated glucose control.
    • Previous limitations in glucose infusion rates required investigation.

    Purpose of the Study:

    • To evaluate the efficacy of the Biostator GCIIS in achieving and maintaining specific circulating glucose levels.
    • To assess the stability of glucose clamps under various glycemic conditions.
    • To identify and address limitations in the Biostator GCIIS system for extended glucose clamping.

    Main Methods:

    • Utilized the Biostator GCIIS for automated glucose clamping in healthy subjects.
    • Established hypoglycemic (42 mg/dl), euglycemic (86 mg/dl), and hyperglycemic (142 mg/dl) clamp levels.

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  • Administered a constant insulin infusion (0.1 U/kg/h).
  • Employed a supplementary glucose infusion to overcome Biostator limitations for euglycemic and hyperglycemic clamps.

    • Main Results:

    • Achieved satisfactory glucose clamps for 120 minutes across all target levels.
    • Reported coefficients of variation for blood glucose: 7.06% (hypoglycemic), 5.9% (euglycemic), and 6.1% (hyperglycemic).
    • Overcame occasional technical issues like catheter blood flow interruption and minor discrepancies with reference glucose measurements.

    Conclusions:

    • The Biostator GCIIS is effective for maintaining stable glucose levels during experimental clamps.
    • The system demonstrates robustness despite minor technical challenges.
    • The findings support the use of Biostator GCIIS for controlled glucose infusion studies.