A quick and simple method for serum HDL determination permitting establishment of an atherogenic index

The present report describes a simple method of differential flocculation for the various lipoproteins. The method, derived from Seidel, allows estimation of serum HDL (high density lipoproteins) as well as determination of the HDL divided by LDL + VLDL ratio. Using Technicon apparatus, total serum lipoprotein flocculation was measured with a dextran solution, and flocculation of LDL + VLDL (low and very low density lipoproteins) was determined with a heparinate solution. The difference between these two measurements reflected the serum HDL level. Results for several verification tests confirmed the method's reliability and showed it to correlate satisfactorily with other quantity determination techniques.