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Ultrastructural morphometry of isolated cells: methods, models and applications

T M Mayhew, F H White

    Pathology, Research and Practice
    |January 1, 1980
    PubMed
    Summary
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    This study explores ultrastructural morphometry in isolated cells, offering a versatile model for pathologists. It details methods for analyzing cell samples, crucial for understanding normal and diseased states.

    Area of Science:

    • Cell Biology
    • Pathology
    • Stereology

    Background:

    • Isolated cells offer a valuable experimental model for ultrastructural morphometry in pathology.
    • A key advantage is the applicability of consistent sampling regimes to both normal and pathologically altered cells.
    • This contrasts with other model systems where sampling may need adjustment based on cellular condition.

    Purpose of the Study:

    • To review methodologies for analyzing isolated cells, including free and cultured cells.
    • To define general concerns in cell analysis, such as preparation, sampling, stereological parameter selection, and error sources.
    • To present generalized and specific morphometric models for cell types like mononuclear phagocytes and lymphocytes.

    Main Methods:

    • Review of preparative techniques for isolated cells.

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  • Discussion of sampling strategies suitable for morphometric analysis.
  • Application of stereological principles to define and analyze cellular parameters.
  • Development of generalized and specific morphometric models for cell analysis.

    • Main Results:

    • Presentation of a generalized morphometric model applicable to various isolated cell types.
    • Detailed descriptions of specific models developed for mononuclear phagocytes and lymphocyte families.
    • Illustrative results from stereological analyses using these models are provided.

    Conclusions:

    • Isolated cells provide a robust and adaptable model system for ultrastructural morphometric studies in pathology.
    • Standardized methodologies and stereological approaches enhance the reliability of cell analysis.
    • The presented models facilitate detailed ultrastructural investigations of specific cell populations.