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Antigen-specific receptor molecules isolated from murine T lymphocytes
Hapten-specific receptor material can be isolated from sensitized murine T and B lymphocytes by the use of hapten-coupled nylon discs. The structural element shared between T and B cell receptors is the variable region of immunoglobulin heavy chains (VH), while known constant immunoglobulin domains appear not to be part of the T cell receptor molecule. The rules governing VH expression (i.e. NPb-idiotype expression) in the B and the T cell compartments are, however, different. Allotype-linked VH (idiotype) expression on T cell molecules was used in genetic reconstitution experiments to prove that the material under study is an endogenous T cell product. With the data available it seems reasonable to view the T cell molecules as representing surface receptors for antigen although this point needs further investigation.
Hapten-specific receptor material can be isolated from sensitized murine T and B lymphocytes by the use of hapten-coupled nylon discs. The structural element shared between T and B cell receptors is the variable region of immunoglobulin heavy chains (VH), while known constant immunoglobulin domains appear not to be part of the T cell receptor molecule. The rules governing VH expression (i.e. NPb-idiotype expression) in the B and the T cell compartments are, however, different. Allotype-linked VH (idiotype) expression on T cell molecules was used in genetic reconstitution experiments to prove that the material under study is an endogenous T cell product. With the data available it seems reasonable to view the T cell molecules as representing surface receptors for antigen although this point needs further investigation.