Immunofluorescence technique for rapid diagnosis of glial tumors

A technique for rapid diagnosis of astroglial and ependymal glial neoplasms is presented. Frozen sections are processed for immunofluorescence using anti-serum raised against the glial fibrillary acidic (GFA) protein. The entire procedure requires 10--15 minutes. The technique is useful for the rapid identification of neoplasms of astroglial and ependymal lineage.