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Two-step leukocyte migration in agarose technique

G Agrup, B Källén, O Nilsson

    International Archives of Allergy and Applied Immunology
    |January 1, 1980
    PubMed
    Summary
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    This study presents an enhanced leukocyte migration assay for improved sensitivity and reproducibility. The modified technique effectively measures immune cell responses to antigens, offering advantages over previous methods.

    Area of Science:

    • Immunology
    • Cellular Immunology
    • Leukocyte Biology

    Background:

    • The Clausen technique is a standard method for assessing leukocyte migration.
    • Modifications are needed to improve sensitivity and reproducibility.

    Purpose of the Study:

    • To describe a two-step modification of the Clausen technique for leukocyte migration under agarose.
    • To evaluate the enhanced technique's sensitivity, reproducibility, and applicability in immune response studies.

    Main Methods:

    • A two-step incubation process involving antigen exposure and cell washing.
    • Migration tests performed on fresh leukocytes using pre-incubated cells.
    • Assessment of inhibition and enhancement of target cell migration.

    Main Results:

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    • Pre-incubation with antigen significantly reduced target leukocyte migration.
    • Inhibition was observed with cells cultured without antigen for up to 3 days.
    • Cells cultured with antigen showed consistent inhibition up to 1 week, with optimal results at 4 days.
    • Cycloheximide treatment dose-dependently blocked the inhibitory effect.
    • Sensitized patients showed antigen-specific migration inhibition or enhancement based on concentration.

    Conclusions:

    • The modified leukocyte migration assay is more sensitive and reproducible than the original Clausen technique.
    • The technique is suitable for studies with limited cell availability.
    • This method provides a valuable tool for studying cellular immune responses and antigen sensitivity.