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Detection of virus particles by electron microscopy with polyacrylamide hydrogel
Journal of Clinical Pathology
|May 1, 1980
Summary
Lyphogel offers a sensitive, economical, and rapid method for concentrating virus particles from samples for electron microscopy. This technique preserves virus morphology, presenting a viable alternative to ultracentrifugation.
Area of Science:
- Virology
- Microscopy
- Biotechnology
Background:
- Electron microscopy requires concentrated samples for effective virus particle visualization.
- Traditional concentration methods like ultracentrifugation can be time-consuming and material-intensive.
Purpose of the Study:
- To evaluate Lyphogel as a method for concentrating virus particles from clinical specimens.
- To compare the sensitivity and efficiency of Lyphogel with ultracentrifugation.
Main Methods:
- Lyphogel concentration was performed on 100 faecal and urine samples.
- Results were compared in parallel with samples concentrated using ultracentrifugation.
- Virus particle morphology was assessed after concentration by both methods.
Main Results:
- Lyphogel demonstrated comparable sensitivity to ultracentrifugation for virus particle concentration.
- Lyphogel proved to be economical, simple, and rapid to use.
- The Lyphogel method required less sample material and preserved virus morphology.
Conclusions:
- Lyphogel is an effective and advantageous alternative to ultracentrifugation for virus particle concentration.
- The method's simplicity and efficiency make it suitable for routine diagnostic laboratories.
- Lyphogel facilitates high-quality electron microscopic examination of viruses.