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Related Experiment Videos

Temporal changes in islet polymerized and depolymerized tubulin during biphasic insulin release

M L McDaniel, C G Bry, R W Homer

    Metabolism: Clinical and Experimental
    |August 1, 1980
    PubMed
    Summary

    Glucose stimulates insulin release through dynamic changes in polymerized tubulin within pancreatic islets. This study reveals tubulin polymerization shifts correlate with both phases of glucose-induced insulin secretion.

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    Area of Science:

    • Cell Biology
    • Endocrinology
    • Molecular Biology

    Background:

    • Insulin secretion from pancreatic islets is a complex process.
    • The role of cytoskeletal dynamics, particularly tubulin, in insulin release is not fully understood.
    • Biphasic insulin release suggests distinct regulatory mechanisms.

    Purpose of the Study:

    • To investigate the temporal correlation between tubulin polymerization and biphasic glucose-induced insulin release.
    • To examine the effect of cytochalasin B on tubulin polymerization and insulin secretion.

    Main Methods:

    • Colchicine binding assay to quantify polymerized and depolymerized tubulin in islets.
    • Incubation of islets at various time points (2.5, 5.5, 10.5, 30.5 min) following glucose stimulation.

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  • Evaluation of cytochalasin B's effect on tubulin equilibrium at 16.5 mM glucose.

    • Main Results:

    • A significant increase in polymerized tubulin (24% to 33%) was observed at 2.5 min, coinciding with the first phase of insulin release.
    • Polymerized tubulin levels decreased, correlating with the decline of the first phase release.
    • Second phase insulin release was associated with tubulin polymerization shifts.
    • Cytochalasin B enhanced polymerized tubulin and insulin release simultaneously.

    Conclusions:

    • Dynamic changes in polymerized tubulin are temporally linked to the rapid events of biphasic insulin release.
    • Tubulin polymerization plays a crucial role in regulating glucose-stimulated insulin secretion.
    • Cytochalasin B's effect on insulin release is mediated, in part, by modulating tubulin polymerization.